Campus Event Calendar
Event Entry
What and Who
Interfering with proteins - statistical challenges of functional cellular assays
Wolfgang Huber
DKFZ-Heidelberg
Talk
AG 1, AG 2, AG 3, AG 4, AG 5
MPI Audience
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Date, Time and Location
Tuesday, 13 July 2004
16:00
-- Not specified --
46.1 - MPII
024
Saarbrücken
Abstract
Systematic cell-based functional assays are a new high-throughput technology
that consist of a system to willfully manipulate the expression level of one
or a certain set of genes in cells, a means to monitor the perturbation,
and a
means to monitor the effect of the perturbation. This can be the
expression or
activation state of another protein, detectable by specific antibodies
with a
fluorescent tag, or morphological changes in the cells. The perturbation can
be caused by overexpression (transfection) or knock-down (RNAi).
The data are interesting as a basis for causally implicating genes and
proteins with pathways or with phenotypes. Measurements are quantitative and
can be obtained on single cells, in large numbers.
The talk will give an overview over the technology, statistical problems of
the 'low-level' interpretation of the data, and mathematical modeling
challenges in integrating them with other sources of information such as
microarray studies.
that consist of a system to willfully manipulate the expression level of one
or a certain set of genes in cells, a means to monitor the perturbation,
and a
means to monitor the effect of the perturbation. This can be the
expression or
activation state of another protein, detectable by specific antibodies
with a
fluorescent tag, or morphological changes in the cells. The perturbation can
be caused by overexpression (transfection) or knock-down (RNAi).
The data are interesting as a basis for causally implicating genes and
proteins with pathways or with phenotypes. Measurements are quantitative and
can be obtained on single cells, in large numbers.
The talk will give an overview over the technology, statistical problems of
the 'low-level' interpretation of the data, and mathematical modeling
challenges in integrating them with other sources of information such as
microarray studies.
Contact
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Ruth Schneppen-Christmann, 07/07/2004 14:11 -- Created document.