| ECCB 2002 Poster sorted by: Author | Number Next | Previous poster (in order of the view you have selected) |
Title: E.coli Heat Shock Response Control Mechanism in Silicon Analysis | P69 |
Hu, Bin; Matsuzaiki,Yuri; Tomita, Masaru; Hiroki, Kitano hubin@sfc.keio.ac.jp Institute for Advanced Biosciences |
| While heat shock response has been studied for decades, little is known about the control mechanism of this process. Based on Kurata's model, we constructed a new computational model to analyze the control mechanism involved in this process at a system level. Our model can reflect the dynamics of major heat shock proteins during and after heat shock. We believe that E.coli heat shock response is an example of HOT (Highly Optimized Tolerance). While previous studies paid much attention to the feedback regulation, our model shows that to achieve robustness, E.coli uses more than one level of control mechanism. Other mechanisms include partial overlap of DnaJ and DnaK functions, as well as competitive binding associated with activation/ deactivation. Those mechanisms, instead of the negative feedback, may play key roles in heat shock response. This model will be available on ECELL version 2 for further analysis and improvement. |
| [1] Kitano, H., Systems biology: a brief overview. Science, 295(5560): p. 1662-4 (2002). [2] Tomita, M., et al., E-CELL: software environment for whole-cell simulation. Bioinformatics, 15(1): p. 72-84 (1999). [3] Kurata H., E.-S.H., Yi T.-M., Khammash M., Doyle J. Feedback Regulation of the Heat Shock Response in E. coli. in Proceedings of the 40th IEEE Conference on Decision and Control (2001). [4] Gross, C.A., Function and Regulation of the Heat Shock Proteins, in Escherichia coli and Salmonella Cellular and Molecular Biology, N. F.C., Editor. Adobe and Mira Digital Publishing (1999). [5] Tatsuta, T., et al., Evidence for an active role of the DnaK chaperone system in the degradation of sigma(32). FEBS Lett, 478(3): p. 271-5 (2000). [6] Carlson, J.M. and J. Doyle, Complexity and robustness. Proc Natl Acad Sci U S A, 99 Suppl 1: p. 2538-45 (2002). [7] Bhalla, U.S. and R. Iyengar, Emergent properties of networks of biological signaling pathways. Science, 283(5400): p. 381-7 (1999). |