| ECCB 2002 Poster sorted by: Author | Number Next | Previous poster (in order of the view you have selected) |
Title: XX_frag: design of cDNA sequences for micro-arrays application to comparative Genomic Hybridization | P65 |
Hubans, Christine; Kerckaert, Jean Pierre; Van Hoecke, Marie Pierre hubans@lifl.fr Genopole de Lille, FRANCE |
| The selection of cDNA sequences probes for micro arrays is an essential step of the process, since the quality of data analysis depends on the quality of these probes. There are several possible applications of micro arrays. One of these is CGH (Comparative Genomic Hybridization), used to study structural genomic abnormalities, such as large deletions. In the case of CGH, the required features of the probes are: specificity of the cDNA sequences (to avoid cross-hybridation), specificity of primers (to ensure the production of the right cDNA sequences) if the probes are supposed to be PCR-fragments and regular distribution of the sequences over the genome or over part of it. A manual selection of this probes is very long and tedious, thus we have designed a method for automated selection and we have developped XX_frag a software which select these probes. |
| [1] V.Cheung, M.Morley, F.Aguilar, A.Massini, R.Kucherlapati and G.Childs ,"Making and Reading microarrays", Nature genetics Vol 21,pp 15-19, January 1999. |