MPI-INF D3 Publications: Journal Article: Metabolic high- content screening of {Saccharomyces} cerevisiae reveals superior performance of single gene deletion strains

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Author, Editor(s)

Author(s):

Velagapudi, Vidya R.
Wittmann, Christoph
Lengauer, Thomas
Talwar, Priti
Heinzle, Elmar

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Not MPG Author(s):

Velagapudi, Vidya R.
Wittmann, Christoph
Heinzle, Elmar

BibTeX cite key*:

Lengauer2006e

Title

Title*:

Metabolic high- content screening of Saccharomyces cerevisiae reveals superior performance of single gene deletion strains

Journal

Journal Title*:	Process Biochemistry	Journal's URL:
Download URL for the article:	http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THB-4K7FCB5-1&_user=43521&_coverDate=10%2F31%2F2006&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000004638&_version=1&_urlVersion=0&_userid=43521&md5=08b9ae3313d5cf0c96cfc0fc8d9203c3	Language:	English

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Vol, No, pp, Date

Volume*:	41	Number:	10
Publishing Date:	2006
Pages*:	2170-2179	Number of VG Pages:
Page Start:		Page End:
Sequence Number:		DOI:	10.1016/j.procbio.2006.06.016

Note, Abstract, ©

Note:
(LaTeX) Abstract:	Twenty-seven deletion mutants of Saccharomyces cerevisiae were investigated using a novel method for high-content stoichiometric and kinetic metabolic profiling. Cultivation on glucose and galactose in 96-well microtiter plates with on-line optical sensing of dissolved oxygen was reproducible and identical to shake flask cultivation. At high shaking rate (1020 rpm) there was no oxygen limitation throughout all cultivations whereas at low shaking rate (540 rpm) oxygen uptake rate could be measured during declining dissolved oxygen concentration. This method allows the determination of specific growth rate, biomass yield on carbon substrate and oxygen and ethanol yield on carbon substrate. Maximum specific growth rates were in the same range for both sugars. On glucose the growth was predominantly fermentative with high yield of ethanol, low yield of biomass and low oxygen consumption rate. On galactose respiration was more active with correspondingly lower ethanol yields, higher biomass yields and higher rates of oxygen consumption. Some strains showed unexpectedly high or low growth rates and rates of ethanol production and respiration. Overall the four parameters determined for each mutant on two different carbon sources allowed statistically significant discrimination of all mutants studied.
URL for the Abstract:	http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THB-4K7FCB5-1&_user=43521&_coverDate=10%2F31%2F2006&_rdoc=12&_fmt=summary&_orig=browse&_srch=doc-info(%23toc%235278%232006%23999589989%23633118%23FLA%23display%23Volume)&_cdi=5278&_sort=d&_docanchor=&view=c&_ct=17&_acct=C000004638&_version=1&_urlVersion=0&_userid=43521&md5=545d003ccc3f963626bbae30bdf2e100
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MPG Unit:	Max-Planck-Institut für Informatik

MPG Subunit:	Computational Biology and Applied Algorithmics
Audience:	Expert
Appearance:	MPII WWW Server, MPII FTP Server, MPG publications list, university publications list, working group publication list, Fachbeirat, VG Wort

BibTeX Entry:

@ARTICLE{Lengauer2006e,
AUTHOR = {Velagapudi, Vidya R. and Wittmann, Christoph and Lengauer, Thomas and Talwar, Priti and Heinzle, Elmar},
TITLE = {Metabolic high- content screening of {Saccharomyces} cerevisiae reveals superior performance of single gene deletion strains},
JOURNAL = {Process Biochemistry},
YEAR = {2006},
NUMBER = {10},
VOLUME = {41},
PAGES = {2170--2179},
DOI = {10.1016/j.procbio.2006.06.016},
}

Entry last modified by Anja Becker, 03/11/2008

Edit History (please click the blue arrow to see the details)

	Editor(s) Ruth Schneppen-Christmann	Created 01/10/2007 09:58:01
Revisions 4. 3. 2. 1. 0.	Editor(s) Anja Becker Christine Kiesel Priti Talwar Ruth Schneppen-Christmann Ruth Schneppen-Christmann	Edit Dates 11.03.2008 09:59:17 08.05.2007 21:37:56 02/23/2007 01:49:25 PM 15.02.2007 10:17:05 10.01.2007 10:01:09

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