ECCB 2002 Poster sorted by: Author | Number

Next | Previous poster (in order of the view you have selected)

Title: The moss transcriptome
P132
Rensing, Stefan; Lang, Daniel; Reski, Ralf

stefan.rensing@biologie.uni-freiburg.de, daniel.lang@biologie.uni-freiburg.de
University of Freiburg, Plant Biotechnology

The moss Physcomitrella patens is the only land plant to date that allows gene disruption by a high rate of homologous recombination. The model organism is therefore an important tool to unravel gene/function relationships. Three cDNA collections covering all important steps of the life cycle have been used to prepare three normalized and subtracted cDNA libraries for the purpose of mass sequencing. After production of around 110,000 ESTs a clustered database was built and analysed. The Physcomitrella transcriptome is estimated to contain around 25,000 genes. Whereas those cover around 50% of the Arabidopsis gene content (around 25,500 genes), Physcomitrella possesses more than 11,000 expressed protein coding genes that cannot currently be identified by terms of sequence homology.
[1] Rensing S.A. et al. (2002) The transcriptome of the moss Physcomitrella patens: comparative analysis reveals a rich source of new genes. http://www.plant-biotech.net/ Rensing_et_al_transcriptome2002.pdf